sk n mc neuroblastoma cells  (ATCC)

Journal: ACS Pharmacology & Translational Science

Article Title: Neuropeptide Y Receptor Binding Profiles of Endogenous Ligands in the Presence and Absence of Sodium: A Panoramic View

doi: 10.1021/acsptsci.6c00127

Figure Lengend Snippet: Radioligand displacement curves obtained from competition binding experiments at the different YR subtypes performed in sodium-containing buffer ( DPBS , 153 mM Na + ; A, C, E, and G) and sodium-free buffer ( buffer Ia (Y 4 R) or buffer Ib (Y 1 R, Y 2 R, Y 5 R); B, D, F, and H). Used cells and radioligands: (A,B) SK-N-MC cells, [ 3 H]­UR-MK299 ( DPBS , K d = 0.084 nM, c = 0.1 nM; buffer Ib , K d = 0.41 nM, c = 0.5 nM). (C,D) CHO-hY 2 R cells, [K 4 -[ 3 H]­propionyl]­hPYY ( DPBS , K d = 0.17 nM, c = 0.4 nM; buffer Ib , K d = 0.078 nM, c = 0.12 nM). (E,F) CHO-hY 4 -G qi5 -mtAEQ cells, [ 3 H]­UR-JG102 ( DPBS , K d = 0.11 nM, c = 0.25 nM; buffer Ia , K d = 0.012 nM, c = 0.03 nM). (G,H) HEC-1B-hY 5 R cells, [K 4 -[ 3 H]­propionyl]­hPYY ( DPBS , K d = 14 nM, c = 10 nM), [K 5 -[ 3 H]­propionyl]­His-hPYY ([ 3 H] 1 ) ( buffer Ib , K d = 20 nM, c = 15 nM). Data represent mean values ± SEM of at least three independent experiments performed in triplicate. JNJ: JNJ31020028.

Article Snippet: SK-N-MC neuroblastoma cells (obtained from the American Type Culture Collection, ATCC HTB-10) were maintained in EMEM supplemented with 7.5% FBS (Pan Biotech; Aidenbach, Germany).

Techniques: Binding Assay

Journal: The Journal of Biological Chemistry

Article Title: Cytomegalovirus-encoded immediate early 1 protein perturbs neural progenitor proliferation via interfering with host PML–DISC1 interaction

doi: 10.1016/j.jbc.2026.111269

Figure Lengend Snippet: DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney (HEK) cells co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.

Article Snippet: SK-N-MC human neuroblastoma cells and HEK cells were purchased from ATCC.

Techniques: Transfection, Immunoprecipitation, Mutagenesis, Binding Assay, Construct, Labeling, Comparison